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KMID : 0359319970370020349
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Korean Journal of Veterinary Research
1997 Volume.37 No. 2 p.349 ~ p.358
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Microbilogy , Studies on Molecular Biological and Immunological Diagnosis of Johne´s Disease
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Chang, Y . F
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Abstract
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Mycobacterium paratuberculosis is the etiologic agent of Johne´s disease, a chronic inflammatory bowel syndrome in ruminants. The attempts to control or eradicate the disease were severely hampered by the inadequacies of present diagnostic methods. The first purpose of this study was to detect Johne´s disease out of 577 cows in the province of Kyunggi, Chungchong, Gangweon and the second purpose was to compare the results of non-absorbed ELISA, absorbed ELISA, PCR, and conventional culture methods. The third purpose was to increase diagnostic specificity, accuracy and rapidity.
When non-absorbed ELISA test was conducted with Mycobacterium paratuberculosis antigen, the prevalence of positive was 10.9%. To increase diagnostic specificity, absorbed ELISA test with Mycobacterium phlei was used. In this test, the positive prevalence was 1.7%.
For the specific detection of Mycobacterium paratuberculosis, PCR was applied to bacterial culture obtained from fecal samples of cattle. The DNA sequences derived from IS900 were used to prepare DNA primers for detection and identification of Mycobacterium paratuberculosis by PCR.
PCR for M paratuberculosis isolated from fecal cultures amplified specific target DNA.
PCR was much more rapid than that obtained by conventional culture technique in diagnosis of Johne´s disease.
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